电离辐射通过转化脂质-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

内容可 ：阐述电磁辐射有否可通过转化趋化因子-β（TGF-β）-特异性的上皮-间质匹配 (EMT)来促进癌亚基质的蹂躏移往。用于总量2Gy(60)Coγ线照射源自人类器官的6种癌亚基质，记录与EMT相关的变化，这包括分别利用孔径新技术，亚基质斯塔夫基方具体方法，免疫荧光新技术，残余物试验车和Transwell小室试验车来注意到并检查亚基质组织形态，EMT标识，蹂躏移往并能等。改用酶先以免疫吸附具体方法检查这些癌亚基质里TGF-β亚基技术水平，利用引人注意诱发剂SB431542来评估TGF-β信号移动式在电磁辐射EMT里的起着。经过总量为2Gy照射的癌亚基质里存在间叶亚基质的表达，与假照射组相比其上皮标识减少，间叶亚基质标识减少，同时其蹂躏转移并能增强，TGF-β亚基技术水平也提高。全面找到由A549电磁辐射诱发的EMT可通过对TGF-β信号诱发遭遇反败为胜。这些相比之下TGF-β特异性的EMT在电磁辐射诱发增强癌亚基质蹂躏转移并能里起着关键性。

撰稿人： lizexiu